From: Charles Plessy Date: Wed, 26 Jan 2022 02:52:53 +0000 (+0900) Subject: Café X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=119ee260fbe55da607136399aea0562955bd1d77;p=source.git Café --- diff --git a/biblio/34815308.mdwn b/biblio/34815308.mdwn new file mode 100644 index 00000000..8bb38495 --- /dev/null +++ b/biblio/34815308.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Comprehensive determination of transcription start sites derived from all RNA polymerases using ReCappable-seq."]] +[[!tag library method]] + +Yan B, Tzertzinis G, Schildkraut I, Ettwiller L. + +Genome Res. 2021 Nov 23. doi:10.1101/gr.275784.121 + +Comprehensive determination of transcription start sites derived from all RNA polymerases using ReCappable-seq. + +[[!pmid 34815308 desc="5 µg of total RNAs was decapped with the the yeast scavenger decapping enzyme (yDcpS), and recapped with vaccinia capping enzyme (VCE) and a biotinylated guanosine. Therefore the protocol enriches for capped, triphosphorylated, diphosphorylated, but not monophosphorylated RNAs. A control library made on RNA dephosphorylated with CIP was used to infer if a TSS is driven by Pol II or Pol III. The methyl-triphosphate cap of RN7SK resists to the CIP treatement and causes it to be incorrectly classified Pol II."]]