From: Charles Date: Wed, 2 Dec 2020 03:41:35 +0000 (+0900) Subject: Café X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=1062d7a46de11e176eef7e55c48da7c460097b49;p=source.git Café --- diff --git a/biblio/10.1101_2020.09.08.286724.mdwn b/biblio/10.1101_2020.09.08.286724.mdwn new file mode 100644 index 00000000..9cea5b64 --- /dev/null +++ b/biblio/10.1101_2020.09.08.286724.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Cell culture-based shark karyotyping as a resource for chromosome-scale genome analysis"]] +[[!tag bioRxiv cell_culture karyotype]] + +Yoshinobu Uno, Ryo Nozu, Itsuki Kiyatake, Nobuyuki Higashiguchi, Shuji Sodeyama, Kiyomi Murakumo, Keiichi Sato, Shigehiro Kuraku + +bioRxiv 2020.09.08.286724; doi: https://doi.org/10.1101/2020.09.08.286724 + +Cell culture-based shark karyotyping as a resource for chromosome-scale genome analysis + +[[!doi 10.1101/2020.09.08.286724 desc="Primary cell culture of fibroblasts and lymphocytes. Mitogens were used to stimulate lymphocyte growth. “2n = 102 for the whale shark (Rhincodon typus) and zebra shark (Stegostoma fasciatum), and 2n = 106 for the brownbanded bamboo shark (Chiloscyllium punctatum) and whitespotted bamboo shark (C. plagiosum)”. In comparison with teleost cell culture medium, shark cells needed a higher osmolarity and the medium was supplemented with 333 mM urea, 188 mM NaCl and 54 mM trimethylamine N-oxide."]]