From: Charles Plessy Date: Wed, 2 Aug 2017 05:28:56 +0000 (+0900) Subject: Cleanup. X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=0cd6962b773b7cf266e94c5101cb6cb7dd3ba7a8;p=source%2F.git Cleanup. --- diff --git a/biblio/15047890.mdwn b/biblio/15047890.mdwn new file mode 100644 index 00000000..9a59fb36 --- /dev/null +++ b/biblio/15047890.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Gene discovery in genetically labeled single dopaminergic neurons of the retina."]] +[[!tag single_cell library]] + +Proc Natl Acad Sci U S A. 2004 Apr 6;101(14):5069-74. doi:10.1073/pnas.0400913101 + +Gustincich S, Contini M, Gariboldi M, Puopolo M, Kadota K, Bono H, LeMieux J, Walsh P, Carninci P, Hayashizaki Y, Okazaki Y, Raviola E. + +Gene discovery in genetically labeled single dopaminergic neurons of the retina. + +[[!pmid 15047890 desc="Uses SMART7, PCR and multiple aRNA rounds."]] diff --git a/biblio/7760832.mdwn b/biblio/7760832.mdwn new file mode 100644 index 00000000..5001116c --- /dev/null +++ b/biblio/7760832.mdwn @@ -0,0 +1,10 @@ +[[!meta title="An efficient strategy to isolate full-length cDNAs based on an mRNA cap retention procedure (CAPture)."]] +[[!tag cap libraries]] + +Mol Cell Biol. 1995 Jun;15(6):3363-71. + +Edery I, Chu LL, Sonenberg N, Pelletier J. + +An efficient strategy to isolate full-length cDNAs based on an mRNA cap retention procedure (CAPture). + +[[!pmid desc="An eIF-4E fusion protein is used to bind the caps of RNA/DNA duplexes. RNase A cuts single stranded RNAs, thus separating the complexes from the cDNA if they are not full length."]] diff --git a/biblio/To_Do b/biblio/To_Do index 45debae2..3280279c 100644 --- a/biblio/To_Do +++ b/biblio/To_Do @@ -64,9 +64,6 @@ Uses T3N9 random nonamer amplification rounds (up to 5), after an initial T7dT R 10499525 [single cell] Single cell RT-PCR. -15047890 [single cell] -Uses SMART7, PCR, and multiple aRNA rounds. - 12711698 [amplification] [tracked] Uses T3N9 instead of T7dT during all the RT reactions. No 3' bias. Efficient on degraded RNA. Independant of polyadenylation (useful for non-polyA genes, like histones). @@ -797,9 +794,6 @@ The cleaved DNA molecules are selected by filling of their ends with biotin-dUTP 15156154 [emulsion] Polymerases selected on the ability to amplify their gene despite a 3' mismach in the primers were shown to be able to incorporate a broad range of nucleotide analogs. -7760832 [libraries] -An eIF-4E fusion protein is used to bind the caps of RNA/DNA duplexes. RNase A cuts single stranded RNAs, thus separating the complexes from the cDNA if they are not full length. - 16308152 [amplification] Detailed protocol for Terminal Continuation (TC).