From: Charles Plessy <plessy@riken.jp>
Date: Thu, 19 Oct 2017 01:29:03 +0000 (+0900)
Subject: ActD.
X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=09d4b081ce9f6af83d81281091020f37a01543ad;p=source.git

ActD.
---

diff --git a/tags/reverse_transcription.mdwn b/tags/reverse_transcription.mdwn
index 7d2e930a..04109217 100644
--- a/tags/reverse_transcription.mdwn
+++ b/tags/reverse_transcription.mdwn
@@ -1,4 +1,12 @@
 [[!meta title="pages tagged reverse transcription"]]
 
-[[!inline pages="tagged(reverse_transcription)" actions="no" archive="yes"
-feedshow=10]]
+(redaction in progress)
+
+Reverse-transcriptases have a DNA-dependent DNA polymerase activity.
+ - It is utilised in [[template_switching]] methods to add linkers to first-strand cDNAs.
+ - It is also a source of antisense artefacts when the RT makes a second-strand cDNA
+   that is mistaken for a first-strand cDNA.  ActinomycinD inhibits DNA-dependent,
+   but not RNA-dependent polymerase activity and is used to suppress these
+   artefacts ([[Perocchi et al., 2007|biblio/17897965]], [[Kanamori-Katayama et al., 2011|biblio/21596820]]).
+
+[[!inline pages="tagged(reverse_transcription)" actions="no" limit=0]]