From: Charles Plessy Date: Mon, 13 May 2019 02:44:04 +0000 (+0900) Subject: adenylylation X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=08457bfb38c350714e66684bdc6eb2d62ffbe56e;p=source%2F.git adenylylation --- diff --git a/tags/adenylylation.mdwn b/tags/adenylylation.mdwn index a33771e8..3a77ffab 100644 --- a/tags/adenylylation.mdwn +++ b/tags/adenylylation.mdwn @@ -3,7 +3,7 @@ Adenylylation ============= -Adenylylation is the addition of an AMP moiety to a phosphorylated end. In vitro, -it is performed by [[ligases|ligases]]. +Adenylylation is the addition of an AMP moiety to a phosphorylated end. In +vitro, it is performed by ligases. See the [[ligase]] tag for details. [[!inline pages="tagged(adenylylation)" limit=0]] diff --git a/tags/ligase.mdwn b/tags/ligase.mdwn index 0fd14bfc..baa0d3c7 100644 --- a/tags/ligase.mdwn +++ b/tags/ligase.mdwn @@ -2,9 +2,6 @@ Work in progress. -DNA and RNA ligases can be used to adenylylate a substrate; seee the -[[adenylylation]] tag for details. - Ligases are inhibited by high concentrations of ATP ([[Tessier, Brousserau & Vernet, 1986|biblio/3799962]] and many others). @@ -32,4 +29,23 @@ On T4 RNL2: linker to RNA ends, thus prevents unwanted ligation products ([[Viollet et al., 2011|biblio/21722378]]). +DNA and RNA ligases can be used to adenylylate a substrate. + + - [[McLaughlin, Piel and Graeser (1985)|biblio/3978074]] noted that + adenylylated RNA is a better substrate for T4 RNA ligase, and that + adenylylation efficiency after 6h was C » U ~ A > G. + - [[Hoffmann and McLaughlin (1987)|biblio/3299268]] used T4 RNL 1 to + adenylylate RNA, and noted that ssNpR(A) are good acceptors, and pCpN + are good donors. + - [[Ho, Wang, Lima and Shuman (2004)|biblio/14962393]] reported the + use of RNL(1-249) on pre-adenylylated RNA in absence of ATP for + avoiding concatemerisation. + - [[Silverman (2004)|biblio/15037782]] reported the use a complementary + DNA oligonucleotide (leaving 3-4 protruding 5'ribonucleotides) when + adenylylating with T4 RNL 1. + [[!inline pages="tagged(ligase)" limit=0]] +[[!meta title="pages tagged adenylylation"]] + + +[[!inline pages="tagged(adenylylation)" limit=0]]