From: Charles Date: Fri, 21 Feb 2020 04:31:11 +0000 (+0900) Subject: Café X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=0744f5cf2db69c283c4e4ac5be41d1cdfda1585f;p=source.git Café --- diff --git a/biblio/2479023.mdwn b/biblio/2479023.mdwn new file mode 100644 index 00000000..0291af1b --- /dev/null +++ b/biblio/2479023.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Extension of mismatched 3' termini of DNA is a major determinant of the infidelity of human immunodeficiency virus type 1 reverse transcriptase."]] +[[!tag reverse-transcription]] + +Proc Natl Acad Sci U S A. 1989 Nov;86(21):8343-7 doi:10.1073/pnas.86.21.8343 + +Perrino FW, Preston BD, Sandell LL, Loeb LA. + +Extension of mismatched 3' termini of DNA is a major determinant of the infidelity of human immunodeficiency virus type 1 reverse transcriptase. + +[[!pmid 2479023 desc="HIV-1 RT can extend A-mismatches with various efficiencies: A:C >> A:A > A:G."]] diff --git a/tags/reverse_transcription.mdwn b/tags/reverse_transcription.mdwn index f9dea9a2..3555b57d 100644 --- a/tags/reverse_transcription.mdwn +++ b/tags/reverse_transcription.mdwn @@ -127,7 +127,9 @@ nucleotide as a template. ### Reverse-transcriptases tolerate terminal mismatches - - Reported by [[Mizuno et al., 1999|biblio/9973624]]. + - HIV-1 RT can extend A-mismatches with various efficiencies: A:C >> A:A > A:G + ([[Perrino and coll., 1898|biblio/2479023]]). + - Reported by [[Mizuno et al., 1999|biblio/9973624]] for double-mismatches and MMLV. - Utilised in [[Arnaud et al., 2016|biblio/27071605]] to reduce priming or ribosomal or hemoglobin RNA. - Specificity of RT nevertheless increases with reaction temperature. For