From: Charles Plessy <plessy@riken.jp>
Date: Mon, 11 Jun 2018 04:36:37 +0000 (+0900)
Subject: Café
X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=073cf532cae06b66e8b52c1f009597388cae2981;p=source.git

Café
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+[[!meta title="Multiplexed precision genome editing with trackable genomic barcodes in yeast."]]
+[[!tag CRISPR yeast screen method]]
+
+Nat Biotechnol. 2018 Jul;36(6):512-520. doi:10.1038/nbt.4137
+
+Roy KR, Smith JD, Vonesch SC, Lin G, Tu CS, Lederer AR, Chu A, Suresh S, Nguyen M, Horecka J, Tripathi A, Burnett WT, Morgan MA, Schulz J, Orsley KM, Wei W, Aiyar RS, Davis RW, Bankaitis VA, Haber JE, Salit ML, St Onge RP, Steinmetz LM,
+
+Multiplexed precision genome editing with trackable genomic barcodes in yeast.
+
+[[!pmid 29734294 desc="Multiplexed accurate genome editing with short, trackable, integrated cellular barcodes (MAGESTIC) uses plasmids providing homologous recombination arms and expressing CRISPR-Cas9 guides.  Recombination rates are further enhanced by LexA sites on the plasmid recruiting a LexA-Fkh1p fusion protein."]]