From: Charles Plessy Date: Fri, 20 Oct 2017 01:01:50 +0000 (+0900) Subject: T4gp32. X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=06c91c014e6ee4ecbdfcd013e1ee5331ac6ed718;p=source%2F.git T4gp32. --- diff --git a/biblio/15028277.mdwn b/biblio/15028277.mdwn index 829aa676..92c0f44d 100644 --- a/biblio/15028277.mdwn +++ b/biblio/15028277.mdwn @@ -1,3 +1,10 @@ [[!meta title="High-accuracy amplification of nanogram total RNA amounts for gene profiling."]] -[[!tag enzyme ssbp]] +[[!tag reverse_transcription ssbp]] + +Kenzelmann M, Klären R, Hergenhahn M, Bonrouhi M, Gröne HJ, Schmid W, Schütz G. + +Genomics. 2004 Apr;83(4):550-8 doi:10.1016/j.ygeno.2003.09.026 + +High-accuracy amplification of nanogram total RNA amounts for gene profiling. + [[!pmid 15028277 desc="Augment temperature, lower primer concentration, and add T4gp32 to enhance reverse transcription."]] diff --git a/biblio/16461948.mdwn b/biblio/16461948.mdwn index 91c86d6b..6155b261 100644 --- a/biblio/16461948.mdwn +++ b/biblio/16461948.mdwn @@ -1,5 +1,5 @@ [[!meta title="Optimization of in vitro transcription and full-length cDNA synthesis using the T4 bacteriophage gene 32 protein."]] -[[!tag reverse_transcription]] +[[!tag reverse_transcription enzyme ssbp]] J Biomol Tech. 2005 Sep;16(3):239-47 diff --git a/tags/reverse_transcription.mdwn b/tags/reverse_transcription.mdwn index 62a3b8f5..8f0d40b9 100644 --- a/tags/reverse_transcription.mdwn +++ b/tags/reverse_transcription.mdwn @@ -5,7 +5,7 @@ Additives that increase reaction performance: - Actinomycin D ([[Perocchi et al., 2007|biblio/17897965]]). - - T4 bacteriophage gene 32 protein (T4gp32, [[Piché _et al._, 2005|biblio/16461948]]). + - T4 bacteriophage gene 32 protein (T4gp32, [[Kenzelmann _et al._, 2004|biblio/15028277]], [[Piché _et al._, 2005|biblio/16461948]]). Reverse-transcriptases have a DNA-dependent DNA polymerase activity. - It is utilised in [[template_switching]] methods to add linkers to first-strand cDNAs.