From: Charles Plessy Date: Thu, 19 Apr 2018 02:30:43 +0000 (+0900) Subject: more X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=06039258ea99828182c79f3b2a42e8191c0ac091;p=source.git more --- diff --git a/tags/reverse_transcription.mdwn b/tags/reverse_transcription.mdwn index 14fe42d7..2f7c1952 100644 --- a/tags/reverse_transcription.mdwn +++ b/tags/reverse_transcription.mdwn @@ -22,7 +22,10 @@ _(redaction in progress)_ ### Terminal desoxynucleotidyl transferase (TdT) activity - [[Chen & Patton, 2001|biblio/11252793]] reported a TdT activity for MMLV and - AMV, with a preference for adding As. + AMV, with a preference for adding As. For MMLV, activity reduced abruptly + between 45 and 50 °C. For AMV, it decreased constantly from 25 to 50 °C. + Activity increased with concentration for MMLV. (High-concentration AMV was + not available.) ### Reverse-transcriptases tolerate terminal mismatches