From: Charles Date: Tue, 21 Jul 2020 04:21:13 +0000 (+0900) Subject: Cleanup X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=03cfc8760295d5a294fe6e0a7040102b956b49f7;p=source.git Cleanup --- diff --git a/biblio/12711698.mdwn b/biblio/12711698.mdwn new file mode 100644 index 00000000..aeaffaec --- /dev/null +++ b/biblio/12711698.mdwn @@ -0,0 +1,10 @@ +[[!meta title="A new strategy to amplify degraded RNA from small tissue samples for microarray studies."]] +[[!tag reverse_transcription amplification]] + +Xiang CC, Chen M, Ma L, Phan QN, Inman JM, Kozhich OA, Brownstein MJ. + +Nucleic Acids Res. 2003 May 1;31(9):e53. doi:10.1093/nar/gng053 + +A new strategy to amplify degraded RNA from small tissue samples for microarray studies. + +[[!pmid 12711698 desc="Uses T3N9 instead of T7dT during all the RT reactions. No 3' bias. Efficient on degraded RNA. Independant of polyadenylation (useful for non-polyA genes, like histones)."]] diff --git a/biblio/To_Do b/biblio/To_Do index de0a3f83..071d9e24 100644 --- a/biblio/To_Do +++ b/biblio/To_Do @@ -20,12 +20,6 @@ Aminoallyl UTP to labal cRNAs. In contrast to cDNAs, DMSO is required for subseq 12161654 [misc] Fluorescent bar-coded oligos to monitor transcription in vivo. -10499525 [single cell] -Single cell RT-PCR. - -12711698 [amplification] [tracked] -Uses T3N9 instead of T7dT during all the RT reactions. No 3' bias. Efficient on degraded RNA. Independant of polyadenylation (useful for non-polyA genes, like histones). - 14606961 [libraries] The nuclease activity of T7 RNA pol could also show some transcript specificity.