From: Charles Date: Tue, 27 Apr 2021 04:46:50 +0000 (+0900) Subject: Café X-Git-Url: https://source.charles.plessy.org/?a=commitdiff_plain;h=0159053b2f678a11b6915c69981c6c9f5752394b;p=source.git Café --- diff --git a/biblio/33899125.mdwn b/biblio/33899125.mdwn new file mode 100644 index 00000000..d235c3c5 --- /dev/null +++ b/biblio/33899125.mdwn @@ -0,0 +1,10 @@ +[[!meta title="Establishing Sustainable Cell Lines of a Coral, Acropora tenuis."]] +[[!tag coral cell_culture cell_line]] + +Kawamura K, Nishitsuji K, Shoguchi E, Fujiwara S, Satoh N. + +Mar Biotechnol (NY). 2021 Apr 26. doi:10.1007/s10126-021-10031-w + +Establishing Sustainable Cell Lines of a Coral, Acropora tenuis. + +[[!pmid 33899125 desc="Dissociation with “a mixture of trypsin, EDTA, and collagenase”. “Treatment for 1–2 h at 25–28 °C did not complete cell dissociation, but after 3–4 h, only single cells without debris were found in the culture dish.” “[The modular protease] plasmin (2 μg/mL) was effective in maintaining dissociated cells in culture for more than 2 weeks, during which a large number of a new type of cell appeared in the culture dish.” “Aliquots of polyclonal cell population were harvested from the primary 24-well culture plate, diluted fivefold with growth medium containing plasmin, and dispensed into 96-well plates (100 μL/well). Cells in clumps proliferated with a doubling time of 2–3 days.”"]] diff --git a/tags/cell_line.mdwn b/tags/cell_line.mdwn index 72dcb22b..b3df749e 100644 --- a/tags/cell_line.mdwn +++ b/tags/cell_line.mdwn @@ -4,5 +4,6 @@ A few notes that just scratch the surface of a vast field… - [[Echalier and Ohanessian (1969)|biblio/4976834]] reported a culture of _Drosophila_ cells that spontaneously transformed. - [[Schneider's (1972)|biblio/4625067]] report of the establishment of the S2 cell line. + - [[Kawamura and coll (2021)|biblio/33899125]] reported the use of plasmin to establish coral cell lines. [[!inline pages="tagged(cell_line)" limit=0]]