Biblio.

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+[[!meta title="Low-cell-number, single-tube amplification (STA) of total RNA revealed transcriptome changes from pluripotency to endothelium."]]
+[[!tag template_switching reverse_transcription]]
+
+Lee YH, Hsueh YW, Peng YH, Chang KC, Tsai KJ, Sun HS, Su IJ, Chiang PM.
+
+BMC Biol. 2017 Mar 21;15(1):22. doi:10.1186/s12915-017-0359-5
+
+Low-cell-number, single-tube amplification (STA) of total RNA revealed transcriptome changes from pluripotency to endothelium.
+
+[[!pmid 28327113 desc="Poly-A tailing followed by template switching.  Increased dNTPs to 2 mM and Mg2+ to 9 mM to favour terminal addition of nucleotides.  TSO and RT primers are at 1 μM final."]]

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    can extend a linker with the sequence of a small RNA via a template switching
    reaction.  (That is: a sRNA can play the same role as a TS oligonucleotide.)
 
+ - [[Lee et al (2017)|biblio/28327113]] increased the efficiency of template
+   switching non-capped molecules by increasing dNTPs to 2 mM and
+   Mg<sup>2+</sup> to 9 mM.
+
 [[!inline pages="tagged(template_switching)" limit=0]]