Spliced-Leader RNA trans Splicing in a Chordate, Oikopleura dioica, with a Compact Genome.
-[[!pmid 15314184 desc="5′ splice leader (SL) found in 90/158 ESTs containing a start codon. The SL RNA is found downstream of the 5S RNA in at least 40 occurences, and aproximately 2/3 of all the 5S rRNA genes. Its sequence is ACTCATCCCATTTTTGAGTCCGATTTCGATTGTCTAACAG. O. doica is the first chordate where gene operons have been described."]]
+[[!pmid 15314184 desc="5′ splice leader (SL) found in 90/158 ESTs containing a start codon. The SL RNA is found downstream of the 5S RNA in at least 40 occurences, and aproximately 2/3 of all the 5S rRNA genes. Its sequence is ACTCATCCCATTTTTGAGTCCGATTTCGATTGTCTAACAG. O. doica is the first chordate where gene operons have been described. Intercistronic regions are very short (<30 nt)."]]
- O. dioica is the first chordate where gene operons have been described. A
40-nt 5′ [[splice leader|tags/trans-splicing]] (SL) bearing a trimethylated cap is found in some RNAs.
The SL gene is found downstream of the 5S RNA gene, which is repeated multiple
- times in the genome. The 3′ acceptor site has a strong UUU(C/U/A)AG consensus
- ([[Ganot et al., 2004|biblio/15314184]]).
+ times in the genome. The 3′ acceptor site has a strong UUU(C/U/A)AG consensus.
+ Reported intercistronic regions are short (<30 nt) ([[Ganot et al., 2004|biblio/15314184]]).
- The splice leader found in the Norwegian strain by ([[Ganot et al., 2004|biblio/15314184]])
was found indentical in a Japanese strain by ([[Wang and coll., 2015|biblio/26032664]]).
- A study using CAGE found that 39% of annotated gene models are trans-spliced with the