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+[[!meta title="RNA amplification strategies for small sample populations."]]
+[[!tag method amplification template_switching]]
+
+Methods. 2005 Nov;37(3):229-37 doi:10.1016/j.ymeth.2005.09.003
+
+Ginsberg SD
+
+RNA amplification strategies for small sample populations.
+
+[[!pmid 16308152 desc="Detailed protocol for Terminal Continuation (TC)."]]

diff --git a/biblio/To_Do b/biblio/To_Do
index 51e467365069423d1cd1118d2caad4476379f014..7fee431110421187d00149e8a9df00e0e1f9ed57 100644 (file)
--- a/biblio/To_Do
+++ b/biblio/To_Do
@@ -32,9 +32,6 @@ ggcacgcga/cc => C-box for dro hairy.
 10537171
 Full in situ random priming cDNA synthesis
 
-9883850 [amplification]
-3 rounds of T7 RNA amplification (aRNA).
-
 12593794 [general]
 Noise due to RNA extraction is 40 times greater than noise due to replicate hybridisation
 
@@ -539,9 +536,6 @@ Unlike mouse ngn3, zebrafish ngn3 is not expressed in the pancreas (but zebrafis
 16280998 [patents]
 Perpetual-motion machines are not patentable.
 
-16308152 [amplification]
-Detailed protocol for Terminal Continuation (TC).
-
 16224006 [IP]
 20 % of the human coding genome is patented.
 

diff --git a/tags/template_switching.mdwn b/tags/template_switching.mdwn
index 79fa05a2688194e9b42a679a6552085cf1d62a40..370aa198be9054593067c7c18786b7609f48cac4 100644 (file)
--- a/tags/template_switching.mdwn
+++ b/tags/template_switching.mdwn
@@ -2,7 +2,10 @@
 
 (work in progress)
 
- - In the "_terminal continuation_" method, [[Che et al., 2004|biblio/14647400]]
-   essentially do template switching with DNA oligonucleotides ending in `CCC`.
+ - The "_terminal continuation_" method ([[Ginsberg et al.,
+   2002|biblio/12462399]], [[Che et al., 2004|biblio/14647400]]) is essentially
+   a template switching with DNA oligonucleotides ending in `CCC` or `GGG`.  `AAA`
+   and `TTT` were also tested.  An extensive protocol was published in
+   [[Ginsberg, 2005|biblio/16308152]].
 
 [[!inline pages="tagged(template_switching)" limit=0]]