Café

diff --git a/biblio/9049314.mdwn b/biblio/9049314.mdwn
new file mode 100644 (file)
index 0000000..66dad94
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+++ b/biblio/9049314.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Determination of the site of first strand transfer during Moloney murine leukemia virus reverse transcription and identification of strand transfer-associated reverse transcriptase errors."]]
+[[!tag reverse_transcription cap]]
+
+EMBO J. 1997 Feb 17;16(4):856-65 doi:10.1093/emboj/16.4.856
+
+Kulpa D, Topping R, Telesnitsky A.
+
+Determination of the site of first strand transfer during Moloney murine leukemia virus reverse transcription and identification of strand transfer-associated reverse transcriptase errors.
+
+[[!pmid 9049314 desc="“The results presented here support the model that +1 substitutions arise during reverse transcription via non‐templated addition followed by mismatch extension upon strand transfer. Another possibility we considered was that +1G could potentially be templated by the 7‐methyl‐G cap present on mRNAs and viral genomic RNAs (Coffin, 1996). Avian myeloblastosis virus reverse transcriptase can add a cap‐complementary C residue during cDNA synthesis on mRNA in vitro, but not when the RNA has been de‐capped (Volloch et al., 1995). However, studies with purified enzymes and model primer–templates have demonstrated that +1G can arise at template switch junctions in the absence of a 7‐methyl‐G cap (Peliska and Benkovic, 1994), and our detection of +1C mutants demonstrates that not all additions to −ssDNA could be cap‐templated.”"]]

diff --git a/tags/reverse_transcription.mdwn b/tags/reverse_transcription.mdwn
index fe9f0ec249e61453b136ec4475cf8a36150eb03d..8880db016d904ca968358260e9cd26a6a77ce784 100644 (file)
--- a/tags/reverse_transcription.mdwn
+++ b/tags/reverse_transcription.mdwn
@@ -86,6 +86,11 @@ nucleotide as a template.
  - [[Volloch et al, 1995|biblio/8534373]] studied cap transcription (but I could
    not access the article).
 
+ - [[Kulpa, Topping and Telesnitsky, 1997|biblio/9049314]] noted the addition
+   of one G at the position where the 5′ end of the MMLV RNA was
+   reverse-transcribed, with a frequency of 10 %.  They also discussed if it
+   could be that the cap was reverse-transcribed.
+
  - [[Ohtake et al, 2004|biblio/15500255]] synthethised RNAs with A-caps and
    showed that they are reverse-transcribed as Ts.