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diff --git a/biblio/16233792.mdwn b/biblio/16233792.mdwn
index 454035de226b30f6ed5cc00c783f15ad866ccd8c..e0edd5a653f660924af650c50b73d62cff8c60d1 100644 (file)
--- a/biblio/16233792.mdwn
+++ b/biblio/16233792.mdwn
@@ -1,3 +1,10 @@
 [[!meta title="Single-molecule reverse transcription polymerase chain reaction using water-in-oil emulsion."]]
-[[!tag emulsion]]
+[[!tag emulsion reverse_transcription]]
+
+J Biosci Bioeng. 2005 Mar;99(3):293-5. doi:10.1263/jbb.99.293
+
+Nakano M, Nakai N, Kurita H, Komatsu J, Takashima K, Katsura S, Mizuno A.
+
+Single-molecule reverse transcription polymerase chain reaction using water-in-oil emulsion.
+
 [[!pmid 16233792 desc="The emulsion is destroyed by centrifugation after 13 cycles, and then the PCR is restarted for 30 cycles."]]

diff --git a/tags/emulsion.mdwn b/tags/emulsion.mdwn
index df1139ed353ba78223058f8aeb05891ae7d06e77..5e2895f528f7db789073067f00cebcb0e6cabe6a 100644 (file)
--- a/tags/emulsion.mdwn
+++ b/tags/emulsion.mdwn
@@ -4,5 +4,9 @@ How to add contents to droplets ?
 
  - In [[Agresti et al., 2005|biblio/16260754]], small molecules mixed with EtOH or DMSO were added by vortexing.
 
+How to break the droplets ?
+
+ - In [[Nakano _et al._, 2005|biblio/16233792]], by centrifugation (oil: silicone; superfactant: TritonX-100 0.1%).
+ - In [[Agresti et al., 2005|biblio/16260754]], by centrifugation in presence of diethyl ether (oil: mineral; superfactant: TritonX-100 0.1% and Span 80 4.5%).
 
 [[!inline pages="tagged(emulsion)" actions="no" feedshow=0]]