Hier dans le train et aujourd'hui au café.

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+[[!meta title="Sensitive, multiplex and direct quantification of RNA sequences using a modified RASL assay."]]
+[[!tag method ligase transcript_count expression]]
+
+Larman HB, Scott ER, Wogan M, Oliveira G, Torkamani A, Schultz PG
+
+Nucleic Acids Res. 2014 Oct 1;42(14):9146-57. doi: 10.1093/nar/gku636
+
+Sensitive, multiplex and direct quantification of RNA sequences using a modified RASL assay.
+
+[[!pmidi 25063296 desc="Decoy probes to reduce the number of reads for the most highly expressed genes.  Uses T4 Rnl2, which was superior in consistency to splintR.  Ligation validated by capillary sequencing.  When hybridizing oligonucleotides to a target to form a nicked double strand, annealing is more efficient if the 5′ end at the nick is not phosphorylated."]]