# Merci Salim.

diff --git a/biblio/20490471.mdwn b/biblio/20490471.mdwn
new file mode 100644 (file)
index 0000000..9306546
--- /dev/null
+++ b/biblio/20490471.mdwn
@@ -0,0 +1,3 @@
+[[!meta title="An improved one-tube RT-PCR protocol for analyzing single-cell gene expression in individual mammalian cells."]]
+[[!tag PCR method buffer single_cell not_read]]
+[[!pmid 20490471 desc="Cell lysis buffer: RNase inhibitor: 2 U/μl, NaCl: 0.135 M, Tris-HCl (pH 8): 9 mM, DTT: 4.5 mM"]]