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+[[!meta title="Mapping 3D genome organization relative to nuclear compartments using TSA-Seq as a cytological ruler"]]
+[[!tag method sequence_tag nucleus]]
+
+J Cell Biol. 2018 Aug 28. pii: jcb.201807108. doi:10.1083/jcb.201807108
+
+Chen Y, Zhang Y, Wang Y, Zhang L, Brinkman EK, Adam SA, Goldman R, van Steensel B, Ma J, Belmont AS.
+
+Mapping 3D genome organization relative to nuclear compartments using TSA-Seq as a cytological ruler 
+
+[[!pmid 30154186 desc="Tyramide free radicals can label DNA directly.  In TSA-Seq (Tyramide Signal Amplification-Sequencing), HRP is targeted on proteins of interest, biotinylated TSA labels neighboring DNA, and biotin-DNA complexes are purified and sequenced.  Distances between chromosome domains and nuclear speckles is reproducible across experiments. Between peripheral lamina (marked by lamin A and B) and nuclear speckles (marked by SON), an increasing gradient of transcriptional activity is clearly visible."]]