café

diff --git a/tags/reverse_transcription.mdwn b/tags/reverse_transcription.mdwn
index adb34865d06a26344f70d26c5d3873158ab3d8ae..51efdcd8f5acc760d001613deef9e7ebab897fb8 100644 (file)
--- a/tags/reverse_transcription.mdwn
+++ b/tags/reverse_transcription.mdwn
@@ -71,13 +71,9 @@ nucleotide as a template.
    2017b|biblio/28747695]]).
 
 
-### The 5′ cap enhances C-tailing
-
- - [[Schmidt & Mueller, 1999|biblio/10518626]] showed that extra cytosine are
-   more frequently added in presence of the 5′ cap.
+### Reverse-transcription of the 5′ cap.
 
-
-### The 5′ cap is reverse-transcribed
+#### Pro:
 
  - [[Hirzmann et al., 1993|biblio/8346046]] observed the presence of an extra G
    at the 5′ end of cDNA clones, and concluded that the cap can be
@@ -91,6 +87,9 @@ nucleotide as a template.
    reverse-transcribed, with a frequency of 10 %.  They also discussed if it
    could be that the cap was reverse-transcribed.
 
+ - [[Schmidt & Mueller, 1999|biblio/10518626]] showed that extra cytosine are
+   more frequently added in presence of the 5′ cap.
+
  - [[Ohtake et al, 2004|biblio/15500255]] synthethised RNAs with A-caps and
    showed that they are reverse-transcribed as Ts.
 
@@ -100,13 +99,20 @@ nucleotide as a template.
 
  - [[Zhang et al, 2017|biblio/28673998]] published a structure of an RNA-GpppG
    complex that suggests that a m7GpppNm / DNA duplex could form during the
-   reverse-transcription of the cap
+   reverse-transcription of the cap.
+
+#### Con:
 
- - Nevertheless, in [[Dallmeier and Neyts, 2013|biblio/23123427]], where
+ - [[Chenchick and coll., 1998|biblio/Chenchick_1998]] reported that non-capped
+   oligonucleotides were extended with long (1~5) cytosine tails, and that the
+   presene of a cap did not have a “significant influence”.
+
+ - In [[Dallmeier and Neyts, 2013|biblio/23123427]], where
    first-strand cDNAs prepared with a phosphorylated reverse-transcription
    primer were circularised, amplified and sequenced, there is not visible
    evidence for G addition.
 
+
 ### Reverse-transcriptases tolerate terminal mismatches
 
  - Reported by [[Mizuno et al., 1999|biblio/9973624]].