Evolutionary changes in cis and trans gene regulation
PATRICIA J. WITTKOPP, BELINDA K. HAERUM & ANDREW G. CLARK
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-Nature 411, 41 - 42 (03 May 2001); doi:10.1038/35075138
-Lethality and centrality in protein networks
-The most highly connected proteins in the cell are the most important for its survival.
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-Nature \ 430, 88 - 93 (01 July 2004); doi:10.1038/nature02555
-Nature AOP, published online 9 June 2004
-Evidence for dynamically organized modularity in the yeast proteinprotein interaction network
-JING-DONG J. HAN1, NICOLAS BERTIN1, TONG HAO1, DEBRA S. GOLDBERG2, GABRIEL F. BERRIZ2, LAN V. ZHANG2, DENIS DUPUY1, ALBERTHA J. M. WALHOUT1,*, MICHAEL E. CUSICK1, FREDERICK P. ROTH2 & MARC VIDAL1
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1557406
Adds a second round of amplification
14732405 [promoters]
Pax6 has at least two transcription start sites.
-15196954 [enhancers] [review]
-Gene networks.
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15379890 [alternative promoters]
RLM-RACE to identify variants. CHIP to confirm TBP on TATA-less promoters. Luciferase assays to confirm new promoters.
16103215 [misc]
Supports the idea that the only function of some RNA is to have been transcribed.
-16094371 [networks]
-Functional validation.
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16077029 [misc]
Suppressive PCR allows the use of one gene-specific primer, and one random primer. Asymetric cloning is the second safeguard against background.
16141073 [genome]
S/AS pairs usually show positive correlation of their expression.
-16168087 [networks]
-Different collaboration network in different research consortiums.
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16120967 [enzymes]
Apparently, only one of the two head-to-head sites is cut...
11842121 [mining]
Normalisation between print tips.
-16141248 [networks]
-Primary paper for the GeneNT R library. Clustering using direct and shortest-path distances.
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16227618 [mirna]
The intergenic transcripts do not seem to be polymerised by pol IV
15582152 [misc]
No correlation between in situ expression pattern and gene ontology in the brain.
-17470793 [networks]
-[not read] Reshaping of networks to a tree-like structure using renormalisation.
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17405768 [microfluidics]
Cycling 100 times (94°C for 30 s, 55°C for 30 s, and 72°C for 60 s) in 0.1% DDM, x1 Pfu Buffer before use improves product yield.
11328886 [pcr]
Method of normalisation taking PCR efficiency into account.
-17609372 [networks]
-Disease network.
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17550599 [enhancers]
35 % of conserved Drosophila enhancers are transcribed (as detected by tiling arrays).
18195365 [misc]
After 10 weeks, elevated DNA strand break levels are detected in the sperm DNA of mice raised near a highway or a steel factory, but not in controls breezing the same but filtered air.
-16902134 [networks]
-Behavioral graph coloring
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16449203 [enzymes]
2'OMe RNAs can not be polyadenylated in vitro.
18369765 [microfluidics]
Another implementation of the revese-transcription on beads.
-18362361 [networks]
-[not read] Algorithm to transform a time serie into a graph.
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18417536 [enhancers]
First report of a conserved enhancer in a coding sequence ?