Biblio

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+[[!meta title="Capture and Amplification by Tailing and Switching (CATS). An ultrasensitive ligation-independent method for generation of DNA libraries for deep sequencing from picogram amounts of DNA and RNA."]]
+[[!tag template_switching amplification method]]
+
+RNA Biol. 2014;11(7):817-28. doi:10.4161/rna.29304
+
+Turchinovich A, Surowy H, Serva A, Zapatka M, Lichter P, Burwinkel B.
+
+Capture and Amplification by Tailing and Switching (CATS). An ultrasensitive ligation-independent method for generation of DNA libraries for deep sequencing from picogram amounts of DNA and RNA.
+
+[[!pmid 24922482 desc="Pre-treatment by T4 PNK was needed when using circulating plasma RNA, probably because of phosphate or cyclo-phosphates at the 3′ end (caused by RNA cleavage).  RT primer concentration scaled down according to the RNA amoounts (0.1 µM for 1 ng and 0.1 nM for 5 pg).  TSO concentration: 1 µM."]]