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+[[!meta title="Purification of histidine-tagged T4 RNA ligase from E. coli."]]
+[[!tag enzyme ligase]]
+
+Biotechniques. 2002 Dec;33(6):1256-60
+
+Wang QS, Unrau PJ.
+
+Purification of histidine-tagged T4 RNA ligase from E. coli.
+
+[[!pmid 12503310 desc="De-adenylation of T4 RNA ligase is required prior using adenylated substrates. Incubating with pyrophosphate during some purification steps does the job. Commercial T4 usually contains mostly de-adenylated enzyme. Mesuring reaction efficiency without ATP gives adenylation levels."]]

diff --git a/biblio/To_Do b/biblio/To_Do
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--- a/biblio/To_Do
+++ b/biblio/To_Do
@@ -89,9 +89,6 @@ Adenylated RNA is a better substrate for T4 RNA ligase.
 3299268 [enzymes]
 Synthesis of adenylated RNA
 
-12503310 [enzymes]
-De-adenylation of T4 RNA ligase is required prior using adenylated substrates. Incubating with pyrophosphate during some purification steps does the job. Commercial T4 usually contains mostly de-adenylated enzyme. Mesuring reaction efficiency without ATP gives adenylation levels.
-
 9373199 [libraries]
 Oligo-capping to build 5' ou FL cDNA libraries.