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+[[!meta title="Man-made cell-like compartments for molecular evolution."]]
+[[!tag emulsion]]
+
+Nat Biotechnol. 1998 Jul;16(7):652-6 doi:10.1038/nbt0798-652
+
+Tawfik DS, Griffiths AD.
+
+Man-made cell-like compartments for molecular evolution.
+
+[[!pmid 9661199 desc="Only the constructs encoding methylases escape degradation."]]

diff --git a/biblio/To_Do b/biblio/To_Do
index e4a5d1f385d234861220520c1607f6305515cb30..5dc847710b34a191dcba0976a7a1b2fb384a68c4 100644 (file)
--- a/biblio/To_Do
+++ b/biblio/To_Do
@@ -574,9 +574,6 @@ Unlike mouse ngn3, zebrafish ngn3 is not expressed in the pancreas (but zebrafis
 16280998 [patents]
 Perpetual-motion machines are not patentable.
 
-99661199 [emulsion]
-Only the constructs encoding methylases escape degradation.
-
 16308152 [amplification]
 Detailed protocol for Terminal Continuation (TC).
 

diff --git a/tags/emulsion.mdwn b/tags/emulsion.mdwn
index 3de02b1ad706bde4135730f457999a1b625d2877..b72e5ee5322d3c4182d8441ab96cdb2c7dc1e773 100644 (file)
--- a/tags/emulsion.mdwn
+++ b/tags/emulsion.mdwn
@@ -7,16 +7,16 @@ How to add contents to droplets ?
 
 How to break the droplets ?
 
- - In [[Nakano _et al._, 2005|biblio/16233792]], by centrifugation (oil: silicone; superfactant: TritonX-100 0.1%).
- - In [[Agresti et al., 2005|biblio/16260754]], by centrifugation in presence of diethyl ether (oil: mineral; superfactant: TritonX-100 0.1% and Span 80 4.5%).
+ - In [[Nakano _et al._, 2005|biblio/16233792]], by centrifugation (oil: silicone; surfactant: Triton X-100 0.1%).
+ - In [[Agresti et al., 2005|biblio/16260754]], by centrifugation in presence of diethyl ether (oil: mineral; surfactant: Triton X-100 0.1% and Span 80 4.5%).
  - High salt:
-   - In [[Dielh _et al._, 2006|biblio/16791214]], by pipetting up and down in 100 mM NaCl, 1 % SDS, 1 % TritonX-100 and Tris (oil: mineral, superfactant: ABIL WE09).
-   - In [[Kojima _et al._, 2005|biblio/16214800]], by mixing with 1M NaCl, 5 mM Tris-HCl pH 8.0, 0.5 mM EDTA (oil: mineral; superfactant: Sun Soft No. 818SK).
- - Extraction of beads with hexane:oil 1:1, in [[Levy _et al._, 2005|biblio/16131588]] (oil: mineral, superfactant: 5.5% Span 80, 0.5% Tween 80, 0.1 % TritonX-100).
+   - In [[Dielh _et al._, 2006|biblio/16791214]], by pipetting up and down in 100 mM NaCl, 1 % SDS, 1 % Triton X-100 and Tris (oil: mineral, surfactant: ABIL WE09).
+   - In [[Kojima _et al._, 2005|biblio/16214800]], by mixing with 1M NaCl, 5 mM Tris-HCl pH 8.0, 0.5 mM EDTA (oil: mineral; surfactant: Sun Soft No. 818SK).
+ - Extraction of beads with hexane:oil 1:1, in [[Levy _et al._, 2005|biblio/16131588]] (oil: mineral, surfactant: 5.5% Span 80, 0.5% Tween 80, 0.1 % Triton X-100).
 
-Different kinds of superfactants:
+Different kinds of surfactants:
 
- - TritonX-100, Span 80, Tween 80 (many articles),
+ - Span 80 4.5 % and Tween 80 0.5 % (oil: mineral; primary paper: [[Tawfik _et al._, 1998|biblio/9661199]], followed by many others, sometimes adding Triton X-100 and changing the concentrations).
  - ABIL WE09 (polysiloxane–polycetyl–polyethylene glycol copolymer; primary paper: [[Dielh _et al._, 2004|biblio/14990785]])
  - Sun Soft No. 818SK (polyglycerol esters of intersesterified ricinoleic acid; primary paper: [[Kojima _et al._, 2005|biblio/16214800]])
  - DC 5225C Formulation Aid / DC 749 Fluid (Dow Chemical Co.) [[Margulies _et al._, 2005|biblio/16056220]]