Au bureau.

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+[[!meta title="Cell fixation and preservation for droplet-based single-cell transcriptomics."]]
+[[!tag methanol fixation method single_cell]]
+
+Alles J, Karaiskos N, Praktiknjo SD, Grosswendt S, Wahle P, Ruffault PL, Ayoub S, Schreyer L, Boltengagen A, Birchmeier C, Zinzen R, Kocks C, Rajewsky N.
+
+BMC Biol. 2017 May 19;15(1):44. doi:10.1186/s12915-017-0383-5
+
+Cell fixation and preservation for droplet-based single-cell transcriptomics.
+
+[[!pmid 28526029 desc='"For rehydration, cells were either kept on ice after fixation (Fixed) or moved from –80 °C to 4 °C (Fixed 1 or 3 weeks) and kept in the cold throughout the procedure. Cells were pelleted at 1000 to 3000 × g, resuspended in PBS + 0.01% BSA, centrifuged again, resuspended in PBS + 0.01% BSA, passed through a 40- or 35-μm cell strainer, counted and diluted for Drop-seq in PBS + 0.01% BSA as described above. "']]