[[!meta title="CapSelect: a highly sensitive method for 5' CAP-dependent enrichment of full-length cDNA in PCR-mediated analysis of mRNAs."]]
-[[!tag cap methods enzyme]]
+[[!tag cap method enzyme]]
[[!pmid 10518626 desc="In presence of the 5' cap and Mn2+, the SuperScriptII enzyme adds 3-4 extra dC residues to the first strand cDNA."]]
[[!meta title="Increased yield of PCR products by addition of T4 gene 32 protein to the SMART PCR cDNA synthesis system."]]
-[[!tag enzyme methods amplification template_switching]]
+[[!tag enzyme method amplification template_switching]]
[[!pmid 11464524 desc="The T4gp32 protein is directly added to the RNA."]]
[[!meta title="Detection of the 5'-cap structure of messenger RNAs with the use of the cap-jumping approach."]]
-[[!tag cap methods]]
+[[!tag cap method]]
[[!pmid 11713326 desc="Binds an oligonucleotide to the cap using its free diol group, and integrates its sequence to the full-length cDNA using template-switching."]]
[[!meta title="Mapping of transcription start sites by direct sequencing of SMART RACE products."]]
-[[!tag methods transcription cap]]
+[[!tag method transcription cap]]
[[!pmid 12661155 desc="Notes that the detected five prime end is frequently off for one or two bases, and supposes that the presence of the cap interferes with the proper recognition of the terminal nucleotides."]]
[[!meta title="Use of alkyltrimethylammonium bromides for the isolation of ribo- and desoxyribo-nucleic acids."]]
-[[!tag methods purification]]
+[[!tag method purification]]
[[!pmid 14076688 desc="Shows the concentration-dependant solubility of CTAB-DNA salts in NaCl."]]
[[!meta title="The biotin-streptavidin interaction can be reversibly broken using water at elevated temperatures."]]
-[[!tag methods biotin]]
+[[!tag method biotin]]
[[!pmid 15690449 desc="One second at seventy degrees separates biotin from streptavidin."]]
[[!meta title="Exogenous AdoMet and its analogue sinefungin differentially influence DNA cleavage by R.EcoP15I--usefulness in SAGE."]]
-[[!tag enzyme products methods sequence_tags]]
+[[!tag enzyme products method sequence_tags]]
[[!pmid 16026759 desc="Sinefungin promotes cleavage of the DNA molecules even if they contain only one EcoP15I site."]]
[[!meta title="The solution to the streptavidin-biotin paradox: the influence of history on the strength of single molecular bonds."]]
-[[!tag methods biotin]]
+[[!tag method biotin]]
[[!pmid 16169976 desc="The strenght of the bond between biotin and streptavidin takes time to reach its maximum."]]
[[!meta title="Coating of poly(dimethylsiloxane) with n-dodecyl-beta-D-maltoside to minimize nonspecific protein adsorption."]]
-[[!tag lab-chip methods]]
+[[!tag lab-chip method]]
[[!pmid 16175253 desc="Continuous washing is necessary for efficient DDM coating."]]
[[!meta title="Organization and mobility of water in amorphous and crystalline trehalose."]]
-[[!tag methods]]
+[[!tag method]]
[[!pmid 16845422 desc="A structural explanation of the protective properties of trehalose."]]
[[!meta title="Counting low-copy number proteins in a single cell."]]
-[[!tag lab-chip methods]]
+[[!tag lab-chip method]]
[[!pmid 17204646 desc="Uses n-dodecyl beta-D-maltose for cell lysis."]]
[[!meta title="A procedure for highly specific, sensitive, and unbiased whole-genome amplification."]]
-[[!tag thermo-enhancement methods]]
+[[!tag thermo-enhancement method]]
[[!pmid 18832167 desc="Locus bias was suppressed for concentrations higher than 0.3 M."]]
[[!meta title="Accurate whole human genome sequencing using reversible terminator chemistry."]]
-[[!tag methods sequencing sequence_tags]]
+[[!tag method sequencing sequence_tags]]
[[!pmid 18987734 desc="Primary paper for Illumina-Solexa technology. The supplemental information contains the sequence of the oligonucleotides used on the flow cells."]]
[[!meta title="A synthetic snRNA m3G-CAP enhances nuclear delivery of exogenous proteins and nucleic acids."]]
-[[!tag cap methods]]
+[[!tag cap method]]
[[!pmid 19208638 desc="The m3G CAP is a signal of nuclear import for the U snRNP."]]
[[!meta title="Genome-Wide Analysis In Vivo of Translation with Nucleotide Resolution Using Ribosome Profiling."]]
-[[!tag sequence_tags methods]]
+[[!tag sequence_tags method]]
[[!pmid 19213877 desc="Also detects the small “upstream open reading frames” (uORFs). Uses circularisation by ssDNA ligase to avoid the bias of the RNA ligase classicaly used to clone small RNAs."]]
[[!meta title="Experimental discovery of sRNAs in Vibrio cholerae by direct cloning, 5S/tRNA depletion and parallel sequencing."]]
-[[!tag library methods]]
+[[!tag library method]]
[[!pmid 19223322 desc="5S and tRNAs are removed by exposing to complementary DNA oligonucletides and RNAse H, that degrades DNA/RNA hybrids."]]
[[!meta title="A library-based method to rapidly analyse chromatin accessibility at multiple genomic regions."]]
-[[!tag biotin library methods DNase-hypersensitivity]]
+[[!tag biotin library method DNase-hypersensitivity]]
[[!pmid 19251760 desc="Includes a smart step to make sure that PCR templates have a different linker at each end."]]
[[!meta title="Novel method for high-throughput colony PCR screening in nanoliter-reactors."]]
-[[!tag methods]]
+[[!tag method]]
[[!pmid 19282448 desc="Uses alginate gel droplets to perform PCR on isolated colonies."]]
[[!meta title="High-throughput verification of transcriptional starting sites by Deep-RACE."]]
-[[!tag sequence_tags promoters high-throughput methods]]
+[[!tag sequence_tags promoters high-throughput method]]
[[!pmid 19317658 desc="Sequencing of 5' RACE PCR products with second generation sequencers."]]
[[!meta title="Chemically inducible inactivation of protein synthesis in genetically targeted neurons."]]
-[[!tag methods plasticity]]
+[[!tag method plasticity]]
[[!pmid 19474302 desc="Combines the inhibitory function of PKR with a drug-inducible dimerisation domain."]]
[[!meta title="Size fractionation of double-stranded DNA by precipitation with polyethylene glycol."]]
-[[!tag methods purification PEG]]
+[[!tag method purification PEG]]
[[!pmid 236548 desc="At lower concentrations, PEG does not preciptiate smaller DNA molecules."]]
[[!meta title="Thermostabilization and thermoactivation of thermolabile enzyme by trehalose and its application for the synthesis of full length cDNA."]]
-[[!tag thermo-enhancement enzyme methods]]
+[[!tag thermo-enhancement enzyme method]]
[[!pmid 9435224 desc="“Thermoactivated reverse transcriptase displays full activity even at 60 degrees ℃”"]]