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+[[!meta title="Transcriptional neighborhoods regulate transcript isoform lengths and expression levels."]]
+[[!tag yeast synthetic variants]]
+
+Brooks AN, Hughes AL, Clauder-Münster S, Mitchell LA, Boeke JD, Steinmetz LM.
+
+Science. 2022 Mar 4;375(6584):1000-1005. doi: 10.1126/science.abg0162
+
+Transcriptional neighborhoods regulate transcript isoform lengths and expression levels.
+
+[[!pmid 35239377 desc="“a synthetic yeast genome (Sc2.0) was designed to encode a Cre-dependent system known as synthetic chromosome rearrangement and modification by LoxP-mediated evolution (SCRaMbLE), which can generate stochastic genomic rearrangements on demand directly in its genome. These rearrangements occur at 34 base pair (bp)–loxPsym sites inserted 3 bp downstream of the stop codon of all nonessential CDSs.”"]]

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@@ -8,4 +8,6 @@ Sc3.0 roadmap: [[Dai and coll., 2020|biblio/32791980]].
 
 Example of neochromosome synthesis in yeast: [[Postma and coll., 2021|biblio/33423048]].
 
+Artificial genome scrambling in yeast 2.0 with loxPsym sites: [[Brooks and coll, 2022|biblio/35239377]].
+
 [[!inline pages="tagged(synthethic)" limit=0]]