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+[[!meta title="Direct selection of trans-acting ligase ribozymes by in vitro compartmentalization."]]
+[[!tag emulsion biotin selection ribozyme]]
+
+RNA. 2005 Oct;11(10):1555-62. doi:10.1261/rna.2121705
+
+Levy M, Griswold KE, Ellington AD.
+
+Direct selection of trans-acting ligase ribozymes by in vitro compartmentalization.
+
+[[!pmid 16131588 desc="During immunolabelling, biotinylated nucleic acids can diffuse from bead to bead if no blocking of biotin is added."]]

diff --git a/biblio/To_Do b/biblio/To_Do
index 2bb9c61c58f6341fd78c6ad759d9dd3d21b194ec..4984443b1b7c865402e681260a9533046d0259ee 100644 (file)
--- a/biblio/To_Do
+++ b/biblio/To_Do
@@ -589,9 +589,6 @@ As tyramide is converted to a free radical which reacts with neighbouring protei
 12433997 [emulsion]
 Random primers were used to introduce mutations.
 
-16131588 [emulsion]
-During immunolabelling, biotinylated nucleic acids can diffuse from bead to bead in no excess of biotin is added.
-
 11274352 [emulsion]
 No beads. A bacterial cel is used as a subcompartment maintaining gene and proteins together. Evidence for leakage of small molecules at high compartment density.
 

diff --git a/tags/emulsion.mdwn b/tags/emulsion.mdwn
index b2634774740fa2c2a66ab5d616ebc027b2c76ee1..65071cf8d952917e1b9a72c76baf45cd654f6daf 100644 (file)
--- a/tags/emulsion.mdwn
+++ b/tags/emulsion.mdwn
@@ -11,6 +11,7 @@ How to break the droplets ?
  - High salt:
    - In [[Dielh _et al._, 2006|biblio/16791214]], by pipetting up and down in 100 mM NaCl, 1 % SDS, 1 % TritonX-100 and Tris (oil: mineral, superfactant: ABIL WE09).
    - In [[Kojima _et al._, 2005|biblio/16214800]], by mixing with 1M NaCl, 5 mM Tris-HCl pH 8.0, 0.5 mM EDTA (oil: mineral; superfactant: Sun Soft No. 818SK).
+ - Extraction of beads with hexane:oil 1:1, in [[Levy _et al._, 2005|biblio/16131588]] (oil: mineral, superfactant: 5.5% Span 80, 0.5% Tween 80, 0.1 % TritonX-100).
 
 Different kinds of superfactants: TritonX-100, Span 80, Tween 80 (many articles), ABIL WE09 ([[Dielh _et al._, 2006|biblio/16791214]] and others), Sun Soft No. 818SK (polyglycerol esters of intersesterified ricinoleic acid [[Kojima _et al._, 2005|biblio/16214800]]).