Expand ; cleanup.

diff --git a/biblio/12019793.mdwn b/biblio/12019793.mdwn
index d75085c4004f46a5743b6ad882f84303fe6026d0..d992b3634ea02cbce78baf99a4da3f8491b9ea2b 100644 (file)
--- a/biblio/12019793.mdwn
+++ b/biblio/12019793.mdwn
@@ -1,3 +1,10 @@
 [[!meta title="Extra-long first-strand cDNA synthesis."]]
-[[!tag thermo-enhancement library]]
+[[!tag thermo-enhancement library enzyme trehalose]]
+
+Biotechniques. 2002 May;32(5):984-5.
+
+Carninci P, Shiraki T, Mizuno Y, Muramatsu M, Hayashizaki Y.
+
+Extra-long first-strand cDNA synthesis.
+
 [[!pmid 12019793 desc="First publication of our laboratory using a mixture of trehalose and sorbitol. T4gp32 does not seem to improve trehalose-enhanced protocols at standard concentrations."]]

diff --git a/biblio/To_Do b/biblio/To_Do
index de9f8d28f8dc7c420d8a4c75290b8b3356855004..d16ba759cda6d4abc4b8d3484a8a29be978e1ed1 100644 (file)
--- a/biblio/To_Do
+++ b/biblio/To_Do
@@ -64,18 +64,12 @@ Single cell RT-PCR.
 12711698 [amplification] [tracked]
 Uses T3N9 instead of T7dT during all the RT reactions. No 3' bias. Efficient on degraded RNA. Independant of polyadenylation (useful for non-polyA genes, like histones).
 
-12188181 [libraries]
-Nuclear integrity preserved during tissue grinding.
-
 12560512 [amplification] [tracked]
 «Semi-linear» Taq polymerase amplification used on the cDNA sample at the labelling step.
 
 14602935 [amplification] [tracked]
 Performs SMART, then exponential PCR, then random-primed klenow labelling.
 
-11414214 [libraries]
-Alternative to homopolymeric priming: the use of GN5 3' overhanging oligls as a template for second strand cDNA synthesis.
-
 12398200 [amplification]
 Advocates a systematic round of RNA amplificatin, becaus it reduces interarray variability.
 
@@ -86,22 +80,12 @@ direct labelling method for big quantities: RNA fragmentation, then T4 RNA ligat
 9373199 [libraries]
 Oligo-capping to build 5' ou FL cDNA libraries.
 
-11337467 [libraries]
-Oligo-capping to study transcription initiation genomewide.
-
-11375929 [libraries]
-Oligo-capping to fine-map transcription start sites.
-
 14606961 [libraries]
 The nuclease activity of T7 RNA pol could also show some transcript specificity.
 
 14704353 [libraries] [tracked]
 T4 DNA ligase used to ligate a double stranded cap-tag.
 
-11730011 [libraries]
-Type IIS restriction digest followed py 3'primer ligation, to rempve the poly A from cDNA.
-
-
 3799962 [enzymes]
 Optimal : Donor/acceptor 5:1; hexammine cobalt chloride 5.0 mM, PEG8000 25%; ATP 100µM. Inhibitors: ATP > 100 µM, MN 2+ > 5mM, NaCl > 20 mM.
 
@@ -126,9 +110,6 @@ cDNAs substracted by their former templates. DNS cleaves DNA from DNA/RNA duplex
 8125298 [libraries]
 Oligo capping primary paper.
 
-11042159 [libraries]
-Biotin-labeled RNA to remove hybridizing cDNA.
-
 12902159 [networks]
 Correlating subnetwork topology, correlation or inverse collrelation, and phase.