--- /dev/null
+[[!meta title="In vitro selection of restriction endonucleases by in vitro compartmentalization."]]
+[[!tag emulsion selection enzyme]]
+
+Nucleic Acids Res. 2004 Jul 6;32(12):e95 doi:10.1093/nar/gnh096
+
+Doi N, Kumadaki S, Oishi Y, Matsumura N, Yanagawa H.
+
+In vitro selection of restriction endonucleases by in vitro compartmentalization.
+
+[[!pmid 15247328 desc="The cleaved DNA molecules are selected by filling of their ends with biotin-dUTP"]]
14500846 [emulsion]
Improvement of STABLE by addtion of one biotinylated end, usage of wheat germ extract for translation, and reduction of the GC content of the streptavidin gene to facilitate PCR.
-15247328 [emulsion]
-The cleaved DNA molecules are selected by filling of their ends with biotin-dUTP
-
15156154 [emulsion]
Polymerases selected on the ability to amplify their gene despite a 3' mismach in the primers were shown to be able to incorporate a broad range of nucleotide analogs.