Café

diff --git a/biblio/31719208.mdwn b/biblio/31719208.mdwn
new file mode 100644 (file)
index 0000000..00673cc
--- /dev/null
+++ b/biblio/31719208.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Definitive demonstration by synthesis of genome annotation completeness."]]
+[[!tag synthethic]]
+
+Jaschke PR, Dotson GA, Hung KS, Liu D, Endy D.
+
+Proc Natl Acad Sci U S A. 2019 Nov 26;116(48):24206-24213. doi:10.1073/pnas.1905990116
+
+Definitive demonstration by synthesis of genome annotation completeness.
+
+[[!pmid 31719208 desc="A øX174 genome where most cryptic ORFs were erased is still viable.  In its initial version, its growth was slower than the wild type, but an in vitro selection isolated a point mutant that recovered the growth phenotype.  Additional experiments suggest that the point mutation affected the translation levels of one of the major ORFs.  A øX174 genome where the major ORFs are mutated is not viable, and only a single cryptic ORF shows detectable levels of expression."]]