Old

diff --git a/biblio/7082652.mdwn b/biblio/7082652.mdwn
new file mode 100644 (file)
index 0000000..f9e911d
--- /dev/null
+++ b/biblio/7082652.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Reversal of T4 RNA ligase."]]
+[[!tag ligase]]
+
+Krug M, Uhlenbeck OC.
+
+Biochemistry. 1982 Apr 13;21(8):1858-64.
+
+Reversal of T4 RNA ligase.
+
+[[!pmid 7082652 desc="T4RNL1 & AMP can remove or exchange 3′ phosphate from RNA molecles.  Thus, 3′-P is not a good blocking group. "]]

diff --git a/biblio/To_Do b/biblio/To_Do
index ed99b9da11135050444d8a4b852b07aa26970ea4..d73e64a9ce7d4829c80dffacf150ab2cb525fd64 100644 (file)
--- a/biblio/To_Do
+++ b/biblio/To_Do
@@ -38,9 +38,6 @@ Uses T3N9 instead of T7dT during all the RT reactions. No 3' bias. Efficient on
 14606961 [libraries]
 The nuclease activity of T7 RNA pol could also show some transcript specificity.
 
-3799962 [enzymes]
-Optimal : Donor/acceptor 5:1; hexammine cobalt chloride 5.0 mM, PEG8000 25%; ATP 100µM. Inhibitors: ATP > 100 µM, MN 2+ > 5mM, NaCl > 20 mM.
-
 14513556 [libraries]
 Hybridises single-stranded tester plasmid and driver PCR product, then purified single strand molecules out of duplexes using hydroxyapatite.
 
@@ -350,9 +347,6 @@ The ultraconserved elements in the Irx clusters are transcription enhancers.
 15899965 [enhancers]
 Out of 126 ultraconserved elements in a melanogaster / A. Gambiae comparison, only 1 is intergenic, the other being mostly ncRNA and exons.
 
-7082652 [enzymes]
-T4RNL1 & AMP can  remove or exchange 3' pNp from RNA molecles.
-
 12429865 [tags]
 Provides an easy access to various statistical tests.
 

diff --git a/tags/ligase.mdwn b/tags/ligase.mdwn
index afc1dd1550256b83b87ce570ff6792d6afda95ad..015ca741c2412510253ca0ea4661f9d3cf909c7b 100644 (file)
--- a/tags/ligase.mdwn
+++ b/tags/ligase.mdwn
@@ -13,7 +13,7 @@ On T4 RNL1:
    Adenylation efficiency after 6h: C >> U ~ A > G ([[McLaughlin et al., 1985|biblio/3978074]]).
  - Also used for single-strand DNA ligation ([[Tessier, Brousserau & Vernet, 1986|biblio/3799962]])
    with PEG and hexamine cobalt chloride (HCC) as additives.
-
+ - Can dephoshporylate RNA 3′ ends ([[Krug & Uhlenbeck, 1982|biblio/7082652]]).  
 
 On T4 RNL2: