18S primers

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+[[!meta title="A method for studying protistan diversity using massively parallel sequencing of V9 hypervariable regions of small-subunit ribosomal RNA genes."]]
+[[!tag eDNA]]
+
+Amaral-Zettler LA, McCliment EA, Ducklow HW, Huse SM.
+
+PLoS One. 2009 Jul 27;4(7):e6372. doi:10.1371/journal.pone.0006372
+
+A method for studying protistan diversity using massively parallel sequencing of V9 hypervariable regions of small-subunit ribosomal RNA genes.
+
+[[!pmid 19633714 desc="Primers contain degenerate sequences and are either universal or eukaryote-based.  Samples from Mount Hope Bay, Massachusetts and Palmer Station, Antarctica."]]
+
+    >18S_1380F eukaryotic 10.1371/journal.pone.0006372
+    CCCTGCCHTTTGTACACAC
+    >18S_1389F universal 10.1371/journal.pone.0006372
+    TTGTACACACCGCCC
+    >18S_1510R eukaryotic 10.1371/journal.pone.0006372
+    CCTTCYGCAGGTTCACCTAC

diff --git a/tags/eDNA.mdwn b/tags/eDNA.mdwn
index 489874099187cdb32d122955feb3b240ec332986..f15996b4f20ac2fd8500cdc9905f9566b96eb1b3 100644 (file)
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+++ b/tags/eDNA.mdwn
@@ -18,6 +18,12 @@
 GCCAGTAGTCATATGCTTGTCT
 >18S_701R 10.1371/journal.pone.0073935
 GGAGCTGGAATTACCGC
+>18S_1380F eukaryotic 10.1371/journal.pone.0006372
+CCCTGCCHTTTGTACACAC
+>18S_1389F universal 10.1371/journal.pone.0006372
+TTGTACACACCGCCC
+>18S_1510R eukaryotic 10.1371/journal.pone.0006372
+CCTTCYGCAGGTTCACCTAC
 ```
 https://doi.org/10.1371/journal.pone.0073935