Café

diff --git a/biblio/10753519.mdwn b/biblio/10753519.mdwn
new file mode 100644 (file)
index 0000000..f6aab4a
--- /dev/null
+++ b/biblio/10753519.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Brachyury (T) expression in embryos of a larvacean urochordate, Oikopleura dioica, and the ancestral role of T."]]
+[[!tag Oikopleura]]
+
+Bassham S and Postlethwait J.
+
+Dev Biol. 2000 Apr 15;220(2):322-32 DOI:10.1006/dbio.2000.9647
+
+Brachyury (T) expression in embryos of a larvacean urochordate, Oikopleura dioica, and the ancestral role of T.
+
+[[!pmid 10753519 desc="O. dioica contains a single ortholog of Brachyury (T, TBXT).  Early expression appears restricted to notochord precursors.  Later, subchordal cells, and even later, hindgut cells also express TBXT.  Translated cDNA sequence was deposited under accession number AAG22592. "]]

diff --git a/tags/Oikopleura.mdwn b/tags/Oikopleura.mdwn
index 0671a7c5612d35e57722dd5b7b68a15546720352..b7b9f7b55a71021496444c6bd2d9c9da3639ece2 100644 (file)
--- a/tags/Oikopleura.mdwn
+++ b/tags/Oikopleura.mdwn
@@ -27,7 +27,10 @@ Food tested in laboratory (totally incomplete list): _Isochrysis galbana_ (5.5
 sp._ (3.5 µm) [[Acuña and Kiefer, 2000|biblio/10.4319_lo.2000.45.3.0608]].
 The diatom _Chaetoceros calcitrans_, often used as a food, can be toxic at high
 concentrations, probably because of the production of biotoxins
-([[Torres-Águila and coll., 2018|biblio/30272001]]).
+([[Torres-Águila and coll., 2018|biblio/30272001]]).  The Postlethwait lab
+has been feeding their animals with (_Dunaliella tertiolecta_, _Isochrysis
+galbana_, _Rhodomonas lens_, _Nanochloropsis sp._, and _Micromonas sp._ (strain Dw-8))
+[[Bassham and Postlethwait (2000)|biblio/10753519]]).
 
 Parasites: _Oodinium pouchetii_ and others.
 
@@ -155,6 +158,9 @@ Genes and pathways
  - _O. dioica_ has 83 homeobox genes, according to [[Edvardsen and coll., 2005|biblio/15649342]].
  - Southern blot analysis suggest the presence of a SCO-spondin gene in _O.
    dioica_ ([[Gobron and coll., 1999|biblio/10197783]]).
+ - A single ortholog of _Brachyury_ (_TBXT_, or _T_) was cloned by [[Bassham
+   and Postlethwait (2000)|biblio/10753519]].  Accession number:
+   [[AF204208|https://www.ncbi.nlm.nih.gov/nuccore/AF204208]].
 
 ### Lost
 
@@ -244,11 +250,14 @@ Development
    rise to the posterior part of the digestive tract (rectum), but not the anus.
    Removal of the trunk suggests that it is not necessary for initiation of the
    migration ([[Kishi and coll, 2014|biblio/25224225]]).
+ - Notochord cell precursors express _Brachyury_ like in other chordates
+   ([[Bassham and Postlethwait (2000)|biblio/10753519]]).
  - The subchordal cell precursors migrate along the right side of the notochord
    in the space that has been filled with endodermal strand cells.  Amputation
    experiments indicate that the posterior portion of the tail is required for
    posterior migration of subchordal cell precursors ([[Kishi and coll,
-   2014|biblio/25224225]]).
+   2014|biblio/25224225]]).  They express _Brachyury_ ([[Bassham and
+   Postlethwait (2000)|biblio/10753519]]).
  - Expression of development genes is retarded by polyunsaturated aldehydes produced
    by diatoms ([[Torres-Águila and coll., 2018|biblio/30272001]]).