]> source.charles.plessy.org Git - source.git/commitdiff
Adapter sequences.
authorCharles Plessy <charles.plessy@oist.jp>
Thu, 13 Dec 2018 06:23:49 +0000 (15:23 +0900)
committerCharles Plessy <charles.plessy@oist.jp>
Thu, 13 Dec 2018 06:23:49 +0000 (15:23 +0900)
biblio/18987734.mdwn
tags/sequencing.mdwn

index 0509d3d55106f3205ff735452f3523dbe57ed6ed..243cf0a6e4b772d6ea96c1963d8fb941bfe4216d 100644 (file)
@@ -1,3 +1,10 @@
 [[!meta title="Accurate whole human genome sequencing using reversible terminator chemistry."]]
 [[!tag method sequencing sequence_tags]]
+
+Nature. 2008 Nov 6;456(7218):53-9. doi: 10.1038/nature07517
+
+Bentley DR, many co-authors, and Smith AJ.
+
+Accurate whole human genome sequencing using reversible terminator chemistry.
+
 [[!pmid 18987734 desc="Primary paper for Illumina-Solexa technology. The supplemental information contains the sequence of the oligonucleotides used on the flow cells."]]
index dcf670dc784aadcdeeca1c9185c224a653a9f3e6..ac52df52054ee1c5d487cb4411076cf4a88ab9a0 100644 (file)
@@ -1,4 +1,19 @@
 [[!meta title="pages tagged sequencing"]]
 
-[[!inline pages="tagged(sequencing)" actions="no" archive="yes"
-feedshow=10]]
+Illumina flowcell adaptors
+--------------------------
+
+Information extracted from the supplemental information of [[Bentley et al., 2008|bilbio/18987734]] (Accurate whole human genome sequencing using reversible terminator chemistry, Nature *456*(7218):53–9)
+
+Single read flowcell
+
+    5´-PSTTTTTTTTTT-(diol)3-AATGATACGGCGACCACCGA-3´
+    5´-PSTTTTTTTTTTCAAGCAGAAGACGGCATACGA-3´
+
+Paired read flowcell
+
+    5´-PS-TTTTTTTTTTAATGATACGGCGACCACCGAGAUCTACAC-3´ (U = 2-deoxyuridine)
+    5´-PS-TTTTTTTTTTCAAGCAGAAGACGGCATACGAGoxoAT-3´  (Goxo = 8-oxoguanine)
+
+
+[[!inline pages="tagged(sequencing)" actions="no" limit=0]]