Café

diff --git a/biblio/30905687.mdwn b/biblio/30905687.mdwn
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@@ -0,0 +1,10 @@
+[[!meta title="Gap junction-dependent coordination of intercellular calcium signalling in the developing appendicularian tunicate Oikopleura dioica."]]
+[[!tag Oikopleura]]
+
+Dev Biol. 2019 Jun 1;450(1):9-22. doi:10.1016/j.ydbio.2019.03.006
+
+Mikhaleva Y, Tolstenkov O, Glover JC.
+
+Gap junction-dependent coordination of intercellular calcium signalling in the developing appendicularian tunicate Oikopleura dioica.
+
+[[!pmid 30905687 desc="Calcium waves observed with GCaMP6.  Each wave is preceded by a “minipeak”.  Two connexins (CxA and CxB) are expressed during embyogenesis.  ”Knockdown of both CxA and CxB mRNA severely disrupts Ca2+ wave propagation and thereby also synchronization.“"]]

diff --git a/tags/Oikopleura.mdwn b/tags/Oikopleura.mdwn
index 3e23f2679139cc5ac89628274e4192415f09bf87..b06aabd606156fe799af2e8623e353598c2329ae 100644 (file)
--- a/tags/Oikopleura.mdwn
+++ b/tags/Oikopleura.mdwn
@@ -178,6 +178,8 @@ Genes and pathways
  - Duplications in the Rab family: Rab5/17, Rab6, Rab7, Rab10, Rab35.  The EF-Rab chimera
    Rab46 is kept ([[Coppola and coll., 2019|biblio/31028425]]).  See below for the losses. 
  - INCENP and Plk1 are duplicated ([[Feng and coll., 2019|biblio/31306061]]).
+ - Two connexins, CxA and CxB are expressed during embyogenesis ([[Mikhaleva,
+   Tolstenkov and Glover 2019|biblio/30905687]]).
 
 ### Lost
 
@@ -286,6 +288,9 @@ Development
    who noted that it is exceptional in invertebrates, and hypothethise that it may
    be caused by neofunctionalisation (house production, ...) or by the small size of
    the genome (doubling the genes would then double the amount of regulatory sequences).
+ - Regular calcium waves are pulsing during embyogenesis.  Their propagation and
+   synchronicity is severly disrupted by the knockdown of the connexins CxA and CxB
+   ([[Mikhaleva, Tolstenkov and Glover 2019|biblio/30905687]]).
  - The tandem _propA_ and _propB_ genes control directly or indirectly _oik41a_
    and proper development of the house-secreting epithelium ([[Mikhaleva et
    al., 2018|biblio/30217597]]).