Old

diff --git a/biblio/163011.mdwn b/biblio/163011.mdwn
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+++ b/biblio/163011.mdwn
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+[[!meta title="A blocked structure at the 5' terminus of mRNA from cytoplasmic polyhedrosis virus."]]
+[[!tag cap not_read]]
+
+Nature. 1975 Jan 31;253(5490):374-5
+
+Furuichi Y, Miura K.
+
+A blocked structure at the 5' terminus of mRNA from cytoplasmic polyhedrosis virus.
+
+[[!pmid 163011 desc="Discovery of the cap."]]

diff --git a/biblio/9373149.mdwn b/biblio/9373149.mdwn
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+[[!meta title="Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library."]]
+[[!tag cap library method]]
+
+Gene. 1997 Oct 24;200(1-2):149-56.
+
+Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S.
+
+Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library.
+
+[[!pmid 9373149 desc="Oligo-capping to build 5′ or FL cDNA libraries."]]

diff --git a/biblio/To_Do b/biblio/To_Do
index 8db5444ddaf9d60a6251ad25e50690e54c0b8156..8028f58cbe6ebdc0e869b0d27a38dad2d12d2f97 100644 (file)
--- a/biblio/To_Do
+++ b/biblio/To_Do
@@ -74,9 +74,6 @@ Advocates a systematic round of RNA amplificatin, becaus it reduces interarray v
 T4 RNA ligase reaction efficiency improved by PEG and pre-adenylation.
 direct labelling method for big quantities: RNA fragmentation, then T4 RNA ligation of biotinylated oligos.
 
-9373199 [libraries]
-Oligo-capping to build 5' ou FL cDNA libraries.
-
 14606961 [libraries]
 The nuclease activity of T7 RNA pol could also show some transcript specificity.
 

diff --git a/tags/cap.mdwn b/tags/cap.mdwn
index 1ca3e1f3584c3f1e3fab005b5e34a9389c94fc39..27bfd58332c43770dcf65c1063be63a513c817e6 100644 (file)
--- a/tags/cap.mdwn
+++ b/tags/cap.mdwn
@@ -6,6 +6,11 @@ Methods for enriching capped RNAs.
 
  - A method similar to oligo-capping was reported by [[Sekine and Kato|biblio/8247743]] in 1993.
 
+ - Oligo-capping [[Suzuki et al., 1999|biblio/9373149]]: dephosphorylate, uncover functional
+   phosphate with decapping enzyme, and ligate RNA adaptor with T4 RNA ligase.
+
+(wip: Fromont-racine et al., Maruyama et al.)
+
  - CapSelect [[Schmidt et al., 1999|biblio/10518626]]: Add a poly-A tail to the first-strand
    cDNA, ligate a double-stranded adapter ending with a single-strand (T)TTTGGG
    overhang, and prime second-strand synthesis.