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+[[!meta title="Functional analysis of a random-sequence chromosome reveals a high level and the molecular nature of transcriptional noise in yeast cells"]]
+[[!tag yeast synthetic]]
+
+Gvozdenov Z, Barcutean Z, Struhl K
+
+Mol Cell. 2023 Apr 24:S1097-2765(23)00254-X. 10.1016/j.molcel.2023.04.010
+
+Functional analysis of a random-sequence chromosome reveals a high level and the molecular nature of transcriptional noise in yeast cells
+
+[[!pmid 37137302 desc="Circular chromosome of 27 kbp comprising 18 kbp of random
+sequences.  “the major class of nucleosomes average 147 bp (range 143–180),
+i.e., the expected size”  “nucleosome occupancy on [the artificial chromosome]
+is 1.42-fold higher than on yeast genomic DNA”  “when compared with yeast
+genomic chromatin, [artificial] chro- matin is characterized by fewer
+[nucleotide-depleted regions], fewer highly positioned nucleosomes, and little
+nucleosome phasing, although nucleosome positioning is nonrandom.”
+“Transcription from all regions of random-sequence DNA leads to similar RNA
+levels that are roughly comparable to that of the majority of mRNA expressed
+from yeast genomic DNA”  “transcription from randomsequence DNA is more
+variable than from genomic DNA”  “decay rates of RNAs expressed from
+randomsequence DNA are 3-fold faster”  “RNAs expressed from [the artificial
+chrmosome] are polyadenylated throughout the entire region of random-sequence
+DNA”  “the choice of poly(A) sites by the cleavage/polyadenylation machinery is
+roughly similar for RNAs expressed from random-sequence and genomic DNAs.” “The
+50 isoform pattern from random-sequence DNA resembles that observed from yeast
+genomic regions not corresponding to 50 UTRs”  “The level of newly synthesized
+RNA from random-sequence DNA is in excellent accord with calculated estimates
+that only 10%–20% of elongating RNA Pol II molecules generate mRNAs, such the
+remaining 80%–90% represent transcriptional noise”"]]

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@@ -12,8 +12,12 @@ Example of neochromosome synthesis in yeast: [[Postma and coll., 2021|biblio/334
 
 Artificial genome scrambling in yeast 2.0 with loxPsym sites: [[Brooks and coll, 2022|biblio/35239377]].
 
+Artificial 18 kbp random chromosome in yeast: [[Gvozdenov, Barcutean and Struhl, 2023|biblio/37137302]].
+
 ## Pathway
 
-Increasing the copy number of the endogenous aldehyde dehydrogenase Hfd1 allowed [[Hu, Yu and Ye, 2022|biblio/35880393]] to obtain the best yield of conversion of retinol to retinoic acid..
+Increasing the copy number of the endogenous aldehyde dehydrogenase Hfd1
+allowed [[Hu, Yu and Ye, 2022|biblio/35880393]] to obtain the best yield of
+conversion of retinol to retinoic acid.
 
 [[!inline pages="tagged(synthethic)" limit=0]]