Avant le café

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+[[!meta title="Genome-scale engineering of Saccharomyces cerevisiae with single-nucleotide precision."]]
+[[!tag CRISPR method screen yeast]]
+
+Nat Biotechnol. 2018 Jul;36(6):505-508. doi:10.1038/nbt.4132
+
+Bao Z, HamediRad M, Xue P, Xiao H, Tasan I, Chao R, Liang J, Zhao H.
+
+Genome-scale engineering of Saccharomyces cerevisiae with single-nucleotide precision.
+
+[[!pmid 29734295 desc="CRISPR–Cas9- and homology-directed-repair (HDR)-assisted genome-scale engineering (CHAnGE) uses plasmids containing HR arms to frameshift or point-mutate precise locations, which are cleaved by Cas9 using a guide RNA expressed by the same plasmid.  Plasmid libraries are made by cloning libraries of long oligonucleotides synthesised on microarrays."]]