--- /dev/null
+[[!meta title="Updating the 97% identity threshold for 16S ribosomal RNA OTUs."]]
+[[!tag eDNA]]
+
+Edgar RC.
+
+Bioinformatics. 2018 Jul 15;34(14):2371-2375. doi:10.1093/bioinformatics/bty113
+
+Updating the 97% identity threshold for 16S ribosomal RNA OTUs.
+
+[[!pmid 29506021 desc="Following a benchmark on V4 regions of the 16S rRNA, proposes to raise the threshold to 99% or to just use sequence variants (also called ZOTUS, Zero-radius OTUs."]]
- A Nextflow pipeline: eDNAFlow [[MousaviāDerazmahalleh and coll., 2021|biblio/10.1111_1755-0998.13356]].
+ - Edgar ([[2018|biblio/29506021]] proposes to raise the threshold to 99% or to
+ just use sequence variants (also called ZOTUS, Zero-radius OTUs.
+
### Published primers
```