café

diff --git a/biblio/23123427.mdwn b/biblio/23123427.mdwn
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+[[!meta title="Simple and inexpensive three-step rapid amplification of cDNA 5' ends using 5' phosphorylated primers."]]
+[[!tag reverse_transcription cap]]
+
+Dallmeier K, Neyts J.
+
+Anal Biochem. 2013 Mar 1;434(1):1-3. doi:10.1016/j.ab.2012.10.031
+
+Simple and inexpensive three-step rapid amplification of cDNA 5' ends using 5' phosphorylated primers.
+
+[[!pmid 23123427 desc="No G-addition observed on mRNAs expressed from a SV40 promoter in Vero-B (African green monkey kidney) cells."]]

diff --git a/tags/reverse_transcription.mdwn b/tags/reverse_transcription.mdwn
index 8880db016d904ca968358260e9cd26a6a77ce784..3b304dcda204d445789b9cd78bdce263bde0ed3f 100644 (file)
--- a/tags/reverse_transcription.mdwn
+++ b/tags/reverse_transcription.mdwn
@@ -102,6 +102,10 @@ nucleotide as a template.
    complex that suggests that a m7GpppNm / DNA duplex could form during the
    reverse-transcription of the cap
 
+ - Nevertheless, in [[Dallmeier and Neyts, 2013|biblio/23123427]], where
+   first-strand cDNAs prepared with a phosphorylated reverse-transcription
+   primer were circularised, amplified and sequenced, there is not visible
+   evidence for G addition.
 
 ### Reverse-transcriptases tolerate terminal mismatches