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+[[!meta title="Direct labeling of RNA with multiple biotins allows sensitive expression profiling of acute leukemia class predictor genes."]]
+[[!tag ligation]]
+
+Nucleic Acids Res. 2004 Jun 17;32(11):e86 doi:10.1093/nar/gnh085
+
+Cole K, Truong V, Barone D, McGall G.
+
+Direct labeling of RNA with multiple biotins allows sensitive expression profiling of acute leukemia class predictor genes.
+
+[[!pmid 15205470 desc="T4 RNA ligase reaction efficiency improved by PEG and pre-adenylation. Direct labelling method for big quantities: RNA fragmentation, then T4 RNA ligation of biotinylated oligos."]]

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@@ -47,10 +47,6 @@ Single cell RT-PCR.
 12711698 [amplification] [tracked]
 Uses T3N9 instead of T7dT during all the RT reactions. No 3' bias. Efficient on degraded RNA. Independant of polyadenylation (useful for non-polyA genes, like histones).
 
-15205470 [libraries] [tracked]
-T4 RNA ligase reaction efficiency improved by PEG and pre-adenylation.
-direct labelling method for big quantities: RNA fragmentation, then T4 RNA ligation of biotinylated oligos.
-
 14606961 [libraries]
 The nuclease activity of T7 RNA pol could also show some transcript specificity.