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diff --git a/biblio/20598146.mdwn b/biblio/20598146.mdwn
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+[[!meta title="Incorporation of non-natural nucleotides into template-switching oligonucleotides reduces background and improves cDNA synthesis from very small RNA samples."]]
+[[!tag template_switching reverse_transcription]]
+
+BMC Genomics. 2010 Jul 2;11:413. doi:10.1186/1471-2164-11-413
+
+Kapteyn J, He R, McDowell ET, Gang DR.
+
+Incorporation of non-natural nucleotides into template-switching oligonucleotides reduces background and improves cDNA synthesis from very small RNA samples.
+
+[[!pmid 20598146 desc="TSO starting with iCiGiC (iso-dC / iso-dG) does not form concatenates because the RTase is inhibited by these nucleotides, therefore it does not reach the end and no extra template switching occurs."]]

diff --git a/tags/template_switching.mdwn b/tags/template_switching.mdwn
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--- a/tags/template_switching.mdwn
+++ b/tags/template_switching.mdwn
@@ -47,6 +47,10 @@ as a replacement for RNA.
  - 3′ phosphate or biotin blocking groups abolish template-switching
    ([[Turchinovich et al (2014)|biblio/24922482]] and others).
 
+ - 5′ iso-dC and iso-dG prevents reverse-transcriptase to reach the end
+   of the TSO, and therefore blocks concatenation
+   ([[Kapteyn et al., 2010|biblio/20598146]]).
+
 ### Effect of TSO concentration
 
  - For the STRT method, [[Zajac et al (2013)|biblio/24392002]] concluded that