Specificity of RT priming at higher temperatures.

diff --git a/tags/reverse_transcription.mdwn b/tags/reverse_transcription.mdwn
index 9e31aa18854fe3ffa6d0a9e5e68cd6a36cc7eaf3..c73e930ff8d8efdc382a5d70abc391cd687b8fc3 100644 (file)
--- a/tags/reverse_transcription.mdwn
+++ b/tags/reverse_transcription.mdwn
@@ -89,6 +89,9 @@ nucleotide as a template.
  - Reported by [[Mizuno et al., 1999|biblio/9973624]].
  - Utilised in [[Arnaud et al., 2016|biblio/27071605]] to reduce priming or
    ribosomal or hemoglobin RNA.
+ - Specificity of RT nevertheless increases with reaction temperature.  For
+   instance, [[Xu and coll., 2019|biblio/30573814]] increased it from
+   ~30 % at 47 °C to ~75 % at 55 °C.
 
 ### Reverse-transcription primers