--- /dev/null
+[[!meta title="Modified 3'-end amplification PCR for gene expression analysis in single cells."]]
+[[!tag single_cell qPCR]]
+
+Heams T, Kupiec JJ.
+
+Biotechniques. 2003 Apr;34(4):712-4, 716.
+
+Modified 3'-end amplification PCR for gene expression analysis in single cells.
+
+[[!pmid 12703294 desc="12703294 Improvement of TPEA method. Gets rid of the nucleus by centrifugation."]]
--- /dev/null
+[[!meta title="Single-cell microarray analysis in hippocampus CA1: demonstration and validation of cellular heterogeneity."]]
+[[!tag single_cell microarray heterogeneity]]
+
+Kamme F, Salunga R, Yu J, Tran DT, Zhu J, Luo L, Bittner A, Guo HQ, Miller N, Wan J, Erlander M.
+
+J Neurosci. 2003 May 1;23(9):3607-15.
+
+Single-cell microarray analysis in hippocampus CA1: demonstration and validation of cellular heterogeneity.
+
+[[!pmid 12736331 desc="Proof of concept and method, from Johnson & Johnson, Acturus, and OmniViz."]]
14606961 [libraries]
The nuclease activity of T7 RNA pol could also show some transcript specificity.
-12736331 [single cell]
-Proof of concept and method, from Johnson & Johnson, Acturus, and OmniViz.
-
14704353 [libraries] [tracked]
T4 DNA ligase used to ligate a double stranded cap-tag.
-12703294 [single cells]
-Improvement of TPEA method. Gets rid of the nucleus by centrifugation.
-
11730011 [libraries]
Type IIS restriction digest followed py 3'primer ligation, to rempve the poly A from cDNA.