Avec du vin

diff --git a/biblio/34099548.mdwn b/biblio/34099548.mdwn
new file mode 100644 (file)
index 0000000..ea8bcc3
--- /dev/null
+++ b/biblio/34099548.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Accurate genomic variant detection in single cells with primary template-directed amplification."]]
+[[!tag amplification method]]
+
+Gonzalez-Pena V, Natarajan S, Xia Y, Klein D, Carter R, Pang Y, Shaner B, Annu K, Putnam D, Chen W, Connelly J, Pruett-Miller S, Chen X, Easton J, Gawad C.
+
+Proc Natl Acad Sci U S A. 2021 Jun 15;118(24):e2024176118. doi:10.1073/pnas.2024176118
+
+Accurate genomic variant detection in single cells with primary template-directed amplification.
+
+[[!pmid 34099548 desc="“primary template-directed amplification (PTA) [...] takes advantage of the processivity, strong strand displacement activity, and low error rate of phi29 polymerase [...] exonuclease-resistant terminators are incorporated into the reaction, creating smaller double-stranded amplification products that undergo limited subsequent amplification. This transforms the reaction from exponential into a quasilinear process with more of the amplification occurring from the primary template.”"]]

diff --git a/tags/amplification.mdwn b/tags/amplification.mdwn
index 798fcbaf55106621f0d61f795259abf5ccb85718..7c8520fee51ace19e27534e889d19513f2096495 100644 (file)
--- a/tags/amplification.mdwn
+++ b/tags/amplification.mdwn
@@ -18,4 +18,9 @@ concentration and annealing temperature) were investigated by [[Dai and coll.,
 complex sample, relatively long ITR (compared to primer length), high
 [annealing temperature], and high concentration of primer should be used.”.
 
+## Other methods
+
+ - Primary Template-directed Amplification (PTA), a kind of MDA with terminators
+   to limit the size of intermediate amplicaons [[Gonzalez-Pena and coll., 2021|biblio/34099548]]
+
 [[!inline pages="tagged(amplification)" limit=0]]