Karyotyping in Ciona

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+[[!meta title="Fluorescent in situ hybridization to ascidian chromosomes."]]
+[[!tag Ciona chromosome]]
+
+Zoolog Sci. 2004 Feb;21(2):153-7 doi:10.2108/zsj.21.153
+
+Shoguchi E1, Ikuta T, Yoshizaki F, Satou Y, Satoh N, Asano K, Saiga H, Nishikata T.
+
+Fluorescent in situ hybridization to ascidian chromosomes.
+
+[[!pmid 14993826 desc="“Embryos at three different developmental stages (32-cell embryos, 64-cell embryos and gastrulae) were used to produce metaphase spreads, although treatment of 32-cell embryos and 64-cell embryos with 0.05% colchicine (Sigma) in seawater caused aggregated and disorganized chromosomes. After treatment with colchicine for 20–30 min, embryos were transferred to a 1.5-ml microfuge tube, to which cold methanol:glacial acetic acid (3:1) fixative was added (chilled on ice). The fixative was changed twice over a period of 1 hr and once after overnight (embryos kept at 4°C). The specimens were then stored in fixative at 4°C.”"]]