STRT.

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+[[!meta title="Characterization of the single-cell transcriptional landscape by highly multiplex RNA-seq."]]
+[[!tag template_switching single_cell method transcriptome tags]]
+
+Islam S, Kjällquist U, Moliner A, Zajac P, Fan JB, Lönnerberg P, Linnarsson S.
+
+Genome Res. 2011 Jul;21(7):1160-7. doi:10.1101/gr.110882.110
+
+Characterization of the single-cell transcriptional landscape by highly multiplex RNA-seq.
+
+[[!pmid 21543516 desc="Single-cell Tagged Reverse Transcription (STRT)"]]

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@@ -13,6 +13,10 @@
    and `TTT` were also tested.  An extensive protocol was published in
    [[Ginsberg, 2005|biblio/16308152]].
 
+ - In the  single-cell tagged reverse transcription (STRT) method,
+   [[Islam et al, 2011|biblio/21543516]]) use template switching and unique
+   molecular identifiers to sequence 5′ ends.  The method is oligo-dT-primed.
+
  - [[Mohr et al (2013)|biblio/23697550]] have shown (Fig 6) that retroviral RTs
    can extend a linker with the sequence of a small RNA via a template switching
    reaction.  (That is: a sRNA can play the same role as a TS oligonucleotide.)
@@ -34,6 +38,11 @@ as a replacement for RNA.
  - [[Arguel et al (2017)|biblio/27940562]] reported similar performance for
    RRR and RRL, using a 5′-focused method similar to nanoCAGE or STRT.
 
+### Effect of TSO concentration
+
+ - For the STRT method, [[Zajac et al (2013)|biblio/24392002]] concluded that
+   1 μM of TSO gave the highest yield.
+
 ### Effect of magnesium, manganese and dNTP concentrations
 
  - [[Schmidt and Mueller, 1999|biblio/10518626]] showed that increasing