RT with low G-addition.

diff --git a/biblio/31363093.mdwn b/biblio/31363093.mdwn
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+[[!meta title="MAPCap allows high-resolution detection and differential expression analysis of transcription start sites."]]
+[[!tag method library reverse-transcription]]
+
+Bhardwaj V, Semplicio G, Erdogdu NU, Manke T, Akhtar A.
+
+Nat Commun. 2019 Jul 30;10(1):3219. doi:10.1038/s41467-019-11115-x
+
+MAPCap allows high-resolution detection and differential expression analysis of transcription start sites.
+
+[[!pmid 31363093 desc="Reports that in their reverse-transcription reaction, G-addition is very low."]]
+
+RT reaction in 20 µL: 10x Buffer 2 μL; dNTP (10 mM) 1 μL → 500 nM final; MgCl2 (25 mM) 4 μL → 5 mM final; DTT (0.1 M) 2 μL → 10 mM final; RNaseOUT (40 U/μl) 1 μL; SSIII (200 U/μL) 1 μL

diff --git a/tags/reverse_transcription.mdwn b/tags/reverse_transcription.mdwn
index 3555b57d7da007e14d3ce60492d5e8a93e1313ac..618d0eca630e10e91c3db17f80edf8868cc1f38e 100644 (file)
--- a/tags/reverse_transcription.mdwn
+++ b/tags/reverse_transcription.mdwn
@@ -65,6 +65,8 @@ to mismatches can be the explanation.
  - [[Mohr and coll, 2013|biblio/23697550]] showed that the Thermostable group
    II intron reverse transcriptase also adds non-templated As.
 
+ - [[Bhardwaj and coll, 2020|biblio/31363093]] report a reverse-transcription
+   with very low G-addition.
 
 ### Templated TdT activity