Café

diff --git a/biblio/10.1101_2020.01.16.905182.mdwn b/biblio/10.1101_2020.01.16.905182.mdwn
new file mode 100644 (file)
index 0000000..a19df76
--- /dev/null
+++ b/biblio/10.1101_2020.01.16.905182.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Simple and efficient measurement of transcription initiation and transcript levels with STRIPE-seq"]]
+[[!tag bioRxiv promoter method]]
+
+bioRxiv Posted January 16, 2020 doi:10.1101/2020.01.16.905182 
+
+Robert A. Policastro, R. Taylor Raborn, Volker P. Brendel and Gabriel E. Zentner
+
+Simple and efficient measurement of transcription initiation and transcript levels with STRIPE-seq
+
+[[!doi 10.1101/2020.01.16.905182 desc="Terminator-treated RNA reverse-transcribed with random N5 primers.  The (biotinylated) TSO is added in the last 5 min of the RT (~40 µM final).  The TSO carries a P5 linker and the RTP a P7 (indexed) linker.  Follows a standard PCR and sequencing.  The YR motif is detected in yeast and K562 cells.  High amounts of rRNA remains in yeast"]]